Influence of Nitrogen Sources on Biomass Growth of Microalgae Chlo­re­lla vulgaris / Golub N.B., Levtun I.I., Tymoshenko E.D. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P.
Background. Microalgae is perspective resource for biodiesel fuel production. However there is no clear data about the best nitrogen source and its contents for high biomass yield and high lipid contents in it.

Objective. Determination of nitrogen sources influence on Chlorella vulgaris biomass yield for its usage as a raw material in biofuels obtaining process.

Methods. Cultivation of microalgae was conducted using mediums containing: CO(NH2)2, NH4NO3, NH4Cl and КNO3 as the nitrogen source. The biomass growth dynamics measurements were based on optical density determination method.

Results. The highest biomass yield occurred in the culture grown with NH4Cl in the medium, and a bit lower yield levels where observed in the medium containing urea, that supports the theory, which stated that more energy is needed to transform nitrogen into restored form. Consumption of ammonium ions leads to rapid decrease of pH level of the medium down to 5. The usage of urea allows keeping medium pH on the level of 7.5.

Conclusions. For maximum yield of Chlorella vulgaris biomass it is necessary to use different nitrogen sources, that allow controlling pH in cultivation process.

Keywords: microalgae; Chlorella vulgaris; nitrogen source; biomass growth; biofuel.

Efficiency of Magnetically Labelled Biosorbent Based on Saccharomyces cerevisiae Yeast for Sewage Treatment / Gorobets S.V., Chyzh Yu.M., Kovalyov O.V., Shpetnyi I.O. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P.
Problems. The problems of magnetically labeled biosorbent (MLBS) associated with a reduction its sorption capacity due to competition of magnetic nano and microparticles and heavy metal ions for binding sites on the surface resulting biosorbent.

Objective. The main goal is to investigate the effectiveness of removal of Cu2+ ions by magnetically labeled biosorbent based Sacharomyces cerevisiae yeast by mixing magnetohydrodynamic (MHD) in crossed electric and magnetic fields and set the optimal technological parameters of the process.

Methods of implementation. The study was conducted at the experimental setup for modification of biosorbent in external electric and magnetic fields.

Results. In experimental studies it was found that the optimum of the pH for the manufacture of magnetically labeled biosorbent by MHD mixing in crossed electric and magnetic fields is pH = 2.5. It is shown that at pH = 2.5 is the maximum extraction of copper cations after 10 minutes at MHD mixing.

Conclusions. The optimal preparation time of MLBS by MHD mixing in crossed electric and magnetic fields, is 6 minutes. We studied magnetic susceptibility of complexes magnetic labels – yeast cell, set the optimum of technological parameters of the process: mixing time, pH value, magnetic field and electric field, studied the stability of the magnetic properties of MLBS.

Keywords: magnetically labelled biosorbent; biosorption; magnetic nanoparticles; crossed electric and magnetic fields.

Potential Producers of Biogenic Magnetic Nanoparticles Among Pathogenic and Opportunistic Microorganisms / Gorobets S.V., Gorobets O.Yu., Butenko K.O. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P.
Problems. The complicated forms of infection in inflammatory processes characterized by rising resistance of microorganisms to antibiotics, are forcing to find new treatments that would prevent development of pathogenic microorganisms, increased local immunity, and thus accelerated regenerative processes.

Objective. Classification of pathogenic and opportunistic microorganisms that may be potential producers of BMN, in terms of the location and properties of BMN using the methods of comparative genomics with the prospect of their subsequent use as the vectors for magnetically targeted delivery of drugs. This will make possible the use of the hyperthermia techniques for removal of pathogenic and opportunistic microorganisms that are capable of biomineralization BMN using for heating the cells directly intracellular BMN of these microorganisms.

Methods of implementation. The methods of paired and multiple sequence alignment were applied using a free access program “BLAST” of National Center for Biotechnology Information.

Results. It was revealed that strains such as E. coli (54115), K. pneumoniae 342, C. perfringens str. 13, P. fluorescens are potential producers of crystalline magnetite and bacteria: S. aureus RF122, S. suis BM407, E. aerogenes KCTC 2190, K. pneumoniae RYC492, P. aeruginosa M18 can be producers of intracellular amorphous BMN. The power of magnetic dipole interaction between BMN of the bacteria and BMN of the tumors is in the range between 107–108 N.

Conclusions. It is explained the neutralization effect of pathogens by the method of magnetic hyperthermia due to the presence BMN, and it was proposed the use for the treatment of inflammatory processes not only antibiotic drugs, but also electromagnetic fields for those microorganisms which are producers of BMN.

It is shown that as vectors for targeted delivery of drugs should be used microorganisms with natural magnetic properties, making method targeted delivery of drugs safer and more efficient, and reduced its cost.

Keywords: magnetic field; biogenic magnetic nanoparticles; inflammation; pathogenic microorganisms; magnetic hyperthermia; targeted delivery.

Obtaining of Transgenic Lettuce Plants, Containing The Recombinant Gene of Thaumatin II Sweet Protein / Kopotun I.P., Scherbak N.L. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P.
Background. At the present, the main direction of genetic engineering research is improving qualities of the crops. The transgenic crops expressing thauII gene successfully combined both the better taste and increasing resistance to fungal pathogens.

Objective. In this study, the recombinant gene of sweettasting protein thaumatin II driven by the CaMV 35S promoter was introduced into lettuce (Lactuca sativa) plants.

Methods. The transformation of cotyledonary explants was achieved via Agrobacterium tumefaciens GV3101. The plasmid vectors used in gene integration experiments also contained selective genes of neomycin phosphotransferase II (nptII ) or phosphinothricin acetyl transferase (bar).

Results. The influence of 0,05 % polyvinylpyrrolidone (10000) on the selective concentration of kanamycin and phosphinotricin in the culture medium were investigated. The optimal concentrations of selective agents for stable transgenic plants selection were used.

Conclusions. Availability of the thaumatin II as well as selective nptII or bar genes was verified by the polymerase chain reaction (PCR). An organoleptic analysis of transgenic lettuce plants did not reveal significant changes in the taste quality. The leaf extracts of these plants will be further tested for antifungal activity.

Keywords: transgenic lettuce; thaumatin II; polyvinylpyrrolidone; kanamycin; phosphinothricin.

Technology Development for the Process of Production Biologically Active Compounds from Callus Culture of Medicinal Plants / Krvavych A.S, Petrini R.O, Novikov V.P. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P.
Background. The widespread use of medicinal plants (MP) for production of biologically active substances is leading to their sharp reduction in their nature. Some of them are rare and endangered species, many plants reduced their long area of growth. One of the actual directions of biotechnology is to obtain the secondary metabolites from plant cells cultured in vitro. The development of biotechnology is based on the study of regularities of callusogenesis and accumulation of secondary metabolites in cell cultures. In this regard, the cultivation of tissues and cells of higher plants in vitro for their industrial use for producing bioactive compounds (BAC) and preserve the gene pool determines the relevance of this study.

Оbjective. The aim of the work is to study of cultivation conditions and development of technology for production of BAC from the callus mass of MP.

Methods. The biotechnological, physical and chemical methods have been used.

Results. The scheme of sterilization of seeds with the highest emergence of the aseptic explants (93 %) and the optimum conditions of process (the medium Murasihe and Skoog, growth regulators, light and temperature regimes have selected for cultivation) has been chosen.

Conclusions. We proposed optimized cultivation conditions and technological scheme for obtaining the BAC from callus mass of Gladiolus imbricatus, which allows to obtain the complex of BAC as an extract of plant cell culture.

Keywords: secondary metabolites; callus mass; Gladiolus imbricatus; cultivation; production.

Development of the Antiseptic Means Compositions on the Basis Of Cytorecifen / Peskova L.О., Goncharova D.O., Molochko M.V., Todosiichuk T.S. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P.
Background. The practical use of bioactive substances becomes complicated by their instability, complication of pretreatment and causing. The use of auxiliary matters is given by possibility to create the comfortable for application prepared form of preparation and correct activity of substance.

Оbjective. The wide spectrum of antimicrobial action of enzyme preparation of Cytorecifen provides grounds to examine him as perspective basis for the soft prepared forms of preparation. Therefore, the purpose of work is to develop composition of the soft prepared form of preparationantiseptic for medicine and veterinary on the basis of Cytorecifen.

Methods. The lytic activity of enzyme complex and it the prepared forms determined a turbidimetric method by the capacity of degradation of suspension of microbe testculture.

Results. It was determined the influence of basic shapegenerating auxiliary materials (proksanol268, PEO400, PEG600, PEG1000, and glycerin), preservatives and anesthetic (miramistin, ofloksacyn, dimeksid, lidocaine) on lytic activity of bacteriolytic enzyme preparation of Cytorecifen. It was proved an absence of substantial negative influence of the chosen auxiliary materials on activity of enzyme, and consequently possibility of their use in composition the prepared forms of preparation.

Conclusions. The composition of antiseptic ointment is offered for veterinary and medical application on the basis of polyoxyethylen, proksanola 268, glycerin, lidocaine and ofloksacyn, containing 40 mg/ml of enzyme preparation of Cytorecifen.

Keywords: antiseptics; prepared forms; Cytorecifen; auxiliary matters.

Study of Antiviral Activity of Recombinant Human Interleukin7 with Various Experimental Models of Hepatitis C Viral / Porva Yu.I., Rybalko S.L., Dyadyun S.T., Lutsenko T.N., Galkin A.Yu., Poholenko Ya.A., Gorbatyuk O.B. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P. Infection
Background. Interleukin7 (IL7) is one of the most important regulatory cytokine of immune system. Given the ability of IL7 to the modulation of T and Bcell responses and Tcell homeostasis we may assume that IL7 not only has the ability to influence the formation of specific immunity and immunodeficiency state, but also inhibit the reproduction of viruses, including hepatitis C virus (HCV).

Objective. Study of antiviral activity of recombinant human IL7 (rIL7) with experimental models of viral hepatitis C infection in vitro on sensitive virus epithelial and lymphoid cells.

Methods. We have used the following inoculated cell cultures: Jurkat, rat neurinoma of gasserian ganglion and bovine kidney. As used surrogate HCV we used virus of bovine diarrhea. To study the antiviral activity different concentrations of rIL7 were injected into the cell culture, producing HCV, and determined virus load. Also we have performed cytological analysis of cells and determined its proliferative activity under influence of rIL7.

Results. It has been shown that rIL7 inhibits surrogate HCV reproduction in in vitro conditions (SS50 – 3 mg/ml, ED50 – 4.7 ng/ml, IS – 640). Highest proliferation of intact Tcells is determined at rIL7 doses 0.3 mg/m and 0.025 mg/ml. rIL7 affected HCV infected cells differently: during the first 3 days the number of cells decreased or did not change, and after 2–3 weeks the number of cells increased almost 2 times. When we injected rIL7 with dose of 6 mg/ml within 3 days we obtained 89% viral inhibition at the 3rd day and 100 % at the 4th day; using the dose of rIL7 0.3 mg/ml the inhibition on the 4th day was 100 %; using dose of rIL7 1.5 mg/ml the inhibition settled at 55 % for 4 days.

Conclusions. As a result of the studies directed towards determining the effect of rIL7 on surrogate HCV reproduction, HCV cDNA producing transfected human Tcells Jurkat, it was showed that rIL7 effectively inhibits virus reproduction.

Keywords: human interleukin7 recombinant; hepatitis C virus; antiviral activity; viral load.

Sensor System for Sulfamethoxazole Detection Based on Molecularly Imprinted Polymer Membranes / Sergeyeva T.A., Piletska E.V., Gorbach L.A., Ivanova A.V., Brovko O.O., El’ska G.V. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P.
Background. Development of sensor systems based on synthetic mimics of biological molecules will provide new effective expressmethods for detection of small organic molecules, including pharmaceuticals, for modern analytical biotechnology.

Objective. An analytical system for highly selective and sensitive detection of sulfamethoxazole based on molecularly imprinted polymer (MIP) membranes is proposed, synthesized using the method of in situ polymerization in a combination with the method of computational modeling.

Methods. Sulfamethoxazole molecules, that were selectively adsorbed by the synthetic binding sites in MIP membranes structure, were visualized due to their ability to form browncolored complexes after reaction with potassium ferricyanide and sodium nitroprusside in alkaline media.

Results. The limit for sulfamethoxazole detection comprised 2 mM, while the linear dynamic range – 2–15 mM, which allows one to detect sulfamethoxazole in pharmaceutical preparations. Stability of the developed MIPbased sensor systems was estimated as at least 6 months, which significantly increases stability of analogous devices based on natural receptors.

Conclusions. Applicability of the developed sensor systems for the analysis of sulfamethoxazole in both model solutions and real samples (commercial pharmaceutical preparations) was proven. The developed systems are characterized with high selectivity, sensitivity, small size and low cost.

Keywords: sensors, sensor systems, molecularly imprinted polymers, membranes, pharmaceuticals, sulfanilamides.

Antimic­robial Spectrum of Antifungal Domestic Drug Esulanum / Starovoitova S.O., Oryabinska L.B., Lubenec V.I. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P.
Background. The first half of the XXIst century is characterized by increasing in the incidence of fungal infections. A wide range of spatial spreading of fungal infections, including dermatophytes and vaginal candidiasis (thrush), which can be explained by intensive migration and changing lifestyles in industrialized countries. This growth was not stopped after the introduction of new antifungal pharmaceuticals, most of which have side effects and are characterized by significant toxicity. In this regard, remains perspective development of new effective nontoxic natural antifungal drugs.

Objective. The study of some potential mechanisms of domestic antimycotic Esulanum such as spectrum antimicrobial activity against grampositive, gramnegative bacteria, microscopic fungi and yeast; influence on morphological characteristics and enzymatic activity of yeast.

Methods. The study of antimicrobial activities was performed by preparation of serial dilutions. Study of the dynamics of cell death under the influence of Esulanum performed on models of culture Candida tropicalis. Cell morphology was studied using a light microscope.

Results. It was shown that the Esulanum has a broadspectrum activity against grampositive and gramnegative bacteria, yeasts and fungies. Minimum inhibitory concentration of Esulanum against yeast (250–500 ug/ml), fungi (62,5–500 ug/ml) grampositive (31,2–250 ug/ml) and gramnegative (62,5–250 ug/ml) bacteria was established. The study of morphological character of Candida tropicalis cells showed that the introduction of Esulanum into the culture medium in the fungicidal concentration led to changes in the morphogenesis of yeast cells, the degree of manifestation of which was due to a temporary agent. The appearance under the influence of the drug bumps and disruptions on the surface of yeast cells, as well as change their shape was probably due to a breach of the normal metabolism of culture, which was confirmed by the experimental data.

Conclusions. The results indicate that Esulanum is a broadspectrum drug, active not only in relation to micromycetes and yeast cultures, but also Grampositive and Gramnegative bacteria. It was found that Esulanum affects the metabolic processes of the fungal cells. During the preclinical studies further research should be aimed at the study of the mechanism of action of the drug on cells of fungal cultures.

Keywords: antifungal drug; Esulanum; mechanism of action; yeast.

Cultivation Features of Higher Basidiomycetes Trametes Zonatus for Liquid Medium / Titova L.O., Klechak I.R. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P.
Background. The basidiomycetes T. zonatus are producers and cellulose oxidative enzymes and polysaccharides with antitumor activity. The feasibility of this study is that the creation of functional food biotechnology from strains of biomass from domestic collections is not enough initial data: fragmentary data on cultivation conditions and culture medium composition, biochemical biomass of T. zonatus.

Оbjective. The aim of the study is to conduct screening of basidiomycetes strains T. zonatus liquid culture medium to determine the effect of the culture medium and the initial pH value of the concentration of biomass T. zonatus 5302, to study the amino acid composition of biomass obtained by submerged T. zonatus 5302 on an culture medium.

Methods. Objects of the research: thirteen basidiomycetes strains T. zonatus IBC. The Standard methods submerged and surface cultivation, conditions cultivation chosen for this strains. Biomass weight was determined by content of dry matter, in the culture broth – areometer method. The source of carbon and nitrogen added to the medium in an amount equimolar respectively to 20 g/dm3 glucose in terms of carbon and 3 g/dm3 NaNO3 in terms of nitrogen. Amino acid composition of proteins after appropriate treatment was studied using amino acid analyzer T339 (“Mikrotechna”, Czech Republic).

Results. According to the results of screening the best strain found by growth characteristics T. zonatus 5302: concentration of biomass – 4.5 g/dm3 productivity biosynthesis biomass – 0.6 g/dm3·day, the economic growth coefficient – 47 %. For culture medium T. zonatus 5302 selected the source of carbon, nitrogen, growth factors, initial pH culture medium.

Conclusions. The biomass T. zonatus 5302, got by submerged cultivation, in the proposed complex culture medium is characterized by a high content of tyrosine and methionine. Index biological value protein strain T. zonatus 5302 amounted to 0.59, which is equal to those of fungi such as Pleurotus ostreatus (0.52) and Ganoderma lucidum (0.48).

Keywords:Basidiomycetes; Тrametes zonatus; screening; submerged cultivation; medium formulation; biomass.

Antiviral and InterferonInducing Action of Aminoethoxydiphenyls / Shemendyuk O.V., Zholner L.G., Zholobak N.M., Spivak N.Ya. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P.
Background. Synthesized compounds 4,4¢bis(2Rethoxy)diphenyl characterized as intercalation into DNA and antiviral activity and ability to induce interferon.

Objective. Determine the antiviral activity and interferon inducer actions of diphenyl derivatives – analogues of Amiksin, in terms of in vitro.

Methods. The work on cell cultures PST and MDBK in conditions in vitro studied antiviral and interferon inducer of the new structural analogues og Amiksin: 4,4¢bis(2dimethylaminoethoxy)diphenyl, 4,4¢bis(24metylpiperydynoethoxy)diphenyl and 4,4¢bis(2[2methyl2(4metylpiperazyn1yl)propil]aminoethoxy)diphenyl.

Results. Diphenyl derivatives shown the ability to inhibit the development of viral cytopathic effect on the model PSTBBC as in prophylactic and therapeutic regimens in compounds. In the model of inoculated cell cultures PST shown that compounds like Amiksin capable of inducing interferon, their advantage is much less toxicity. With the introduction of experimental animals experimental compounds IFN geneactive substances have been better than Amiksin.

Conclusions The results can be considered as derivatives of diphenyl compounds that are able to provide virus protection cells not only due to the activation of interferon production, but other mechanisms, the study of which is the goal of future research.

Keywords: Amiksin; antiviral activity; vesicular stomatitis virus; interferon inducing action; diphenyl derivatives.

Impact of Infraslow Corrosion on the Duration of Transient Processes in Amperometric Gas Sensors / Buket O.I. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P.
Background. The duration of transient processes is an important characteristic of amperometric gas sensors.

Objective. The objective of the study is a determination of nature of the transition processes that increase the time required to establish a reliable signal amperometric sensor and the variation of the time.

Methods. The chronoamperometry techniques for transient processes and determination of their temperature coefficient shows the effect of corrosion on the sensors of standardised series of NTUU “KPI”.

Results. The minimum duration of the transient state is responsible of oxide layers, which is achieved by cyclic current load of the working electrode, including “training” of the electrode with the current on level of infraslow corrosion (slower as 1 micron/year). It is observed periodic variation of the properties of these oxide layers by the infraslow corrosion, which fades with flow of time and without external influence. The interference of these cycles with periodic changes in the external environment may accidentally cause an unexpected slowdown in the currentproducing process.

Conclusions. Cyclic current loads form on the working electrode of amperometric sensor some oxide structures with size up to 10 nm, similar to the socalled nanotehnologіes method of the “bottomup” type. Without such current loads surface oxide layers are sealed, and their resistance increases and inhibits the currentforming process. The direct detection of such structures by electron microscopy requires prior theoretical justification of the research methodology.

Keywords:amperometric sensors; duration of transient’s processes; corrosion of the electrodes; nanostructured oxides.

The Template SolGel Method for Syn­thesis of Tin (IV) Oxide Nanoparticles / Dontsova T.A. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P.
Background. It is known that the presence of templates greatly affects on physical and chemical properties of the synthesized powders. Therefore, investigation of powders synthesis SnO2 in the presence and absence of templates by solgel method are scientific and practical interest.

Objective. The purpose this paper was synthesis of SnO2 nanoparticles in absence and presence of template nonionic type (ethylene glycol and PEG) by solgel method and characterization of obtained powders.

Methods. In paper synthesized the tin (IV) oxide powders by solgel method with and without the use of templates. The ethylene glycol and polyethylene glycol with a molecular weight of 6000 (PEG 6000) was used as templates.

Results. The minimum temperature for crystallization of hydrated tin (IV) oxide powders is defined by thermal analysis. It is found that the heat treatment under these conditions leads to the formation of the polycrystalline powders pure rutile modification with a low degree of crystallinity and with a structure approximated to amorphous state. It is shown that the solgel method produces nanocrystalline (0.8–3.5 nm) and nanoparticulate (10–15 nm) powders of tin oxide (IV), and the use of templatePEG 6000 2– 4 times reduces the size of crystallites. It is shown that the increase in mass of content template PEG6000 from 1 to 10–75 % results in a slight distortion of the crystal lattice of tin oxide (IV). The calculated value of the bandgap for SnO2 is equal to 3,69 eV. This value is in good agreement with literature data.

Conclusions. The use of solgel method allows to obtain of nanoparticle SnO2 powders, and template – significantly smaller crystallite size SnO2 (to 0.8 nm). It is shown that minimum temperature for receiving of crystalline SnO2 is 350 °C.

Keywords: Tin (IV) Oxide; nanoparticles; solgel method; template.

Basic Laws of Elektrobaromembrane of Wastewater Treatment Process from Fe3+ Ions with Obtaining the Alkali, Acid and Hydrogen / Dulneva T.Yu., Kucheruk D.D. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P.
Background. The various methods used to clean the wastewater for the iron removal do not always provide a high degree of purification by ion Fe3+. Therefore, the development of new highly effective methods of treatment of waste water is very important and urgent.

Objective. The study of basic laws of nonreagent elektrobaromembrane process wastewater treatment of Fe3+ ions with the help of a tubular titanium membranecathode to obtain alkali, acid and hydrogen, as well as development on this basis of the principal block scheme of such treatment is considered.

Methods. The Elektrobaromembrane method is used with tubular membranes of titanium as the cathode.

Results. It has been investigated an influence of current density, of operating pressure, duration of the process, the concentration of Fe3+ ions in the initial solution on the delay factor R of the ions, their concentration in the filtrate, specific performance Jv of the membranecathode in water and leachate, pH leachate and recirculating solution. It is shown that for water with a concentration of ions Fe3+ in the range 50–100 mg/dm3 should be used the current density of 150–300 A/m2 at an operating pressure of about 0,1–0,2 MPa.

Conclusions. Based on the results has been developed principal block scheme of water treatment from Fe3+ ions to obtain alkali, acid and hydrogen by elektrobaromembrane method. At the same time can get in 1 hour on 1 m2 of the surface tubular membranecathode 15–60 dm3 of alkali with pH ~ 12; 20–65 dm3 of hydrogen and appropriate amount of acid with pH ~ 2.5.

Keywords: wastewater treatment; elektrobaromembrane method; membrane of titanium; hydroxocomplexes of iron; production of alkali, acid and hydrogen.

The Studies of the Nickel Precipitation Kinetics from Solution / Korchuhanova O.M., Tantsyura E.V. K. Naukovi visti NTUU “КPІ”. – 2015. – N 3. – P.
Background. In the nickelcontaining catalysts production there is the technological stage of the intermediate insoluble nickel compounds. The studies of the nickel precipitation kinetics by the urea from the nickel nitrate solution were carried out that are useful for the design and characteristics calculation of these processes,

Objective. The purpose is in constructing of mathematical model for the nickel precipitation process.

Methods. A thermodynamic evaluation of possible reactions in solution was carried out. In isothermal conditions at the temperatures of 90 and 100 °C the kinetic curves of nickel precipitation from solutions were obtained at different initial concentrations. On the curves several sections were marked: the induction period and the main reaction period that are analyzed by different methods. The dependence of the induction period value from the conditions of the process was analyzed using multiple regression approach. The kinetic equation of first order reaction was chosen for the main period and the temperature dependence of the rate according to Arrhenius equation was determined.

Results. It is established that the value of the induction period depends on the process temperature and the concentration of nickel, the regression equation was obtained. It is shown that the basic section of the precipitation process kinetic curves are described by the equation of the first order at the different initial concentrations of metal in all the investigated temperature range, reaction rate constant of the first order and the activation energy of the process were calculated.

Conclusions. The proposed kinetic model of precipitation that consists of equations system for calculating the time of reaction was obtained. The value of the activation energy of the process indicates a kinetic stage of process tendency.

Keywords: nickel nitrate; urea; kinetics of deposition; the induction period; the order of reaction; mathematical model; time.