Antonenko L.O., Bisko N.A., Mytropolska N.Yu., Klechak I.R.

Boundary Temperatures for Cultivation of Basidiomycetes Mushrooms of Genus Coriolus Quel. in the Surface Culture

P. 7–11.

Fig. 2. Tabl. 3. Refs.: 21 titles.

In this paper, we define the value of high and low boundary temperatures for 33 strains of basidiomycetes mushrooms of Coriolus. We prove the existence of strains variability for C. versicolor, C. zonatus, C. hirsutus depending on a high temperature. In addition, we obtain the research results on strains viability for C. zonatus, C. hirsutus and C. villosus at high temperatures for the first time ever.

Galkin O.Yu., Grygorenko A.A.

Bioethics in Ukraine: from Theory to Practice. Legal, Scientific and Educational Aspects

P. 12–19.

Refs.: 24 titles.

We provide the insight into the background of bioethics and its differentiation at the present stage. Moreover, we consider the approaches to legal regulation in the field of bioethics at the international level and relevant domestic legislation. We also justify that bioethics should be introduced as an academic discipline for training specialists in biology, biotechnology and pharmacy.

Golub N.B., Andruchovets V.M.

Conditions of Formation and Structure of Biofilms

P. 20–26.

Fig. 3. Tabl. 1. Refs.: 28 titles.

We analyze the conditions (temperature, рН, physical and chemical features of substrates structure, a liquid growth medium, a cell surface, mass-transfer processes) which influence the optimal development of a biofilm, including an anode biofilm formed directly on the anode of a microbial fuel cell (МFC). We define that the quality and properties of the biofilm influence an extracellular polymeric matrix and the presence of quorum-sensing between members of microbial community.

Gorobetz S.V., Gorobetz O.Yu., Demjanenko I.V.

Self-Organization of Magnetic Nanoparticles When Giving Magnetic Properties to Yeast Saccharomyces Cerevisiae

P. 27–33.

Fig. 4. Tabl. 1. Refs.: 10 titles.

We develop and conduct the compararative analysis of four evaluation methods of clusters parameters of magnetic nanoparticles. The magnetically-controllable biosorbent based on the S. cerevisiae culture is used as a model object for analyzing the adequacy of these models. Finally, we obtain the evaluation data on the depth of clusters penetration inside the biomembrane, typical sizes of clusters and dispersion of magnetic cluster sizes. Our analysis shows that all four methods can be used for single magnetic clusters. However, for clusters laying in chains with small distances between their centers, the model that takes into account the table meaning of magnetite magnetization can’t be used, because this model doesn’t take into account the nearest neighbors contribution of interaction of magnetic fields dipole with magnetic probe.

Dekhtyarenko N.V., Dugan А.М.

Features of Preparation and Fermentation of Soybean Milk by the Representatives of Lactobacillus sp.

P. 34–39.

Tabl. 4. Refs.: 16 titles.

The first stages of development of new laboratory technology sanitary sour-milk products on the basis by an especial mode cultivated seeds of soy have shown. For preparation soybean milk the soybean flour made according to new national technology ЕСОâ (producer ООО “ЕСО”) has used. The optimum technology of pretreatment of a soybean flour has found with the purpose of subsequent usage as a base for nutrient fermentations mediums. Has established, that milk from soy ЕСО “The present of the sun – Super” as much as possible induce biosynthetic processes for the strains L. delbrueckii subsp. lactis LE and L. rhamnosus LB3.

Dzygun L.P.

Medicinal Xylotrophic Basidiomycetes Laetiporus Sulphureus (Bull.: Fr.) Murill – Perspective Object of Biotechnology

P. 40–49.

Tabl. 5. Refs.: 40 titles.

This paper brings into light the critical review of publications on the chemical composition, action, and the use of compounds isolated from fruit bodies, submerged mycelium and culture liquid of edible basidiomycete Laetiporus sulphureus. In addition, we uncover biologically active compounds revealing the role of L. sulphureus as a substances producer that can be  applied in various fields of industry.

Krupodorova T.A.

The Influence of Temperature Incubation on Vitality of Vegetative Mycelia of Ganoderma applanatum (Pers.) Pat. and G. lucidum (Curtis) P. Karst. stains

P. 50–55.

Tabl. 2. Refs.: 14 titles.

The paper studies the influence of temperature incubation on vitality of vegetative mycelia of 40 mushroom stains of the Ganoderma genus. We determine that the species under study show strains specificity on vitality of vegetative mycelia at their incubation at 33–45 °С. We also establish the critical growth temperature for vegetative mycelia of G. applanatum and G. lucidum stains.

Linovytska V.М., Buchalo А.S., Dugan A.М.

Selection of Conditions of Submerged Cultivation of Grifola frondosa as the Basis for Development of Biotechnology for Obtaining Medicinal Prophylactic Preparations

P. 56–60.

Fig. 8. Refs.: 20 titles.

The paper investigates the influence of sources of carbon and nitrogen, pH and components of nutrient mediums (beer wort, molasses, corn extract, peptone, extract of yeasts) on the accumulation of biomass and exopolysaccharides under cultivation of higher basidiomycetes mushroom of Grifola frondosa (Dicks: Fr.) S.F. Gray. Crucially, we propose complex nutrient mediums with glucose, ammonium nitrate, corn extract and molasses favorable for biosynthesis of exopolysaccharides and biomass.

Manilo M.V., Ar’ev I.A., Lytvynov G.S.

Carbon Nanostructures for Biotechnology: Technologies of Carbon Nanotube Synthesis with Definite Space Properties

P. 61–67.

Fig. 5. Tabl. 4. Refs.: 22 titles.

The study summarizes major achievements in the field of single- and multi-layer carbon nanotube synthesis. We discuss the factors that influence the properties of carbon nanotubes. The method of chemical vapour deposition enables to control geometrics of carbon nanostructures. It is crucial for the target use in medicine biotechnology as a media for neuronal and endothelial cells at production of new effective systems for transportation of drugs and therapeutics, as well as in biosensors miniaturization. Finally, we emphasize the importance of further research and modification of methods for obtaining to determine definite properties of carbon nanotubes.

Marynchenko L.V., Nizhelska O.I., Marynchenko V.O.

Stimulation of Accumulation of Biomass and Fermenting Activity of Saccharomyces cerevisiae Yeast Culture by Extra High Frequency Electromagnetic Irradiation

P. 68–73.

Fig. 1. Tabl. 4. Refs.: 24 titles.

The optimum irradiation parameters of EHF EMR for Saccharomyces cerevisiae M-09 yeast culture are experimentally determined. In the alcoholic brew, fermented with the irradiated yeast, the accumulation of biomass, alcohol and the amount of carbon dioxide emissions increased compared to control. Simultaneously the amount of nonfermented sugars decreased. The yeast vigor for the filtered brew yeast, their a-glucosidase and zymase activity enhanced. The effect of the EHF EMR action on the yeast culture remained for three generations and gradually weakened, which can be connected with Saccharomyces cerevisiae adaptation to the influence of external factors.

Polishchuk V.Yu., Malanyuk M.I., Dugan A.M.

Morphological and Cultural and Biosynthetic Properties of Eremothecium Ashbyi Guill.

P. 74–78.

Fig. 5. Refs.: 18 titles.

This paper considers the morphological and cultural properties of ascomycetes Eremothecium ashbyi F-340, a producer of riboflavin in various nutrient mediums. We also demonstrate the accretion of fungal biomass, the level of its riboflavin accumulation and the consumption of carbohydrates of nutrient mediums. Through experiments conducted, we determine that using the medium which contains wort in its composition is the most reasonable. Moreover, the riboflavin level in this medium is 2,8 times higher than in other mediums.

Rezenchuk O.E., Povodzinskiy V.M., Shybetskyi V.Yu.

Classification and Analysis of Fermenters with Pneumatic Mixing

P. 79–84.

Fig. 6. Refs.: 7 titles.

The analysis of constructions of fermenters with energy supply by compressed gas in view of features of hydrodynamics and math transfering in them, also there was made an observing of evolutionary progress of modern constructions. Based on this information, there was made the classification of fermenters with pneumatic mixing with the main idea was to find possible ways of designing and building such kind of equipment.

Samarukha I.A.

Investigation of Mediatorless Bioelectrogenes Process by Association of Anaerobic Microorganisms. Biotechnological Indices

P. 85–89.

Fig. 2. Tabl. 1. Refs.: 14 titles.

We develop the approaches to classification of technological parameters of effectiveness evaluation of the mediatorless exoelectrogenesis process based on the system features. Moreover, we propose the ways of calculating the efficiency of biotechnological process parameters, namely the biomass growth (total and specific), the rate of substrate consumption (efficiency of wastewater purification), Coulomb efficiency and COD balance. Finally, we experimentally justify the possibility of biotechnological electricity generation using the association of anaerobic microorganisms on the laboratory equipment of the mediatorless microbial fuel cell.

Todosiychuk T.S., Strelets T.I., Konopatska S.V.

Increasing the Microbial Pathogen Stability as the Factor of the Development of Novel Antiseptic Means

P. 90–97.

Refs.: 35 titles.

This paper demonstrates the role of major mechanisms of resistance and additional factors in the process of increasing the stability of microbial pathogenes. We describe the approaches to prevention of microorganisms resistance formation as well as ways and perspectives of developing novel antiseptic means. Among promising directions of new antiseptics development are preparations that specifically inhibit the mechanisms of cell¢s growth (QS-system etc.), bacteriophage substances and its derivatives, modified bacteriolytic enzymes.

Trokhymenko O.P., Zholner L.G., Kutsaev P.V., Kutsik V.M.

Modeling of the Technological Process for Live Rotavirus Vaccine Production

P. 98–104.

Fig. 4. Refs.: 18 titles.

We simulate the industrial technology for rotavirus vaccine, based on the strain of group A rotavirus with G1P[8] genotype, circulating among people in Ukraine. We propose to use this strain of rotavirus as the industrial strain. Through successive passages, it is adapted to cultivation conditions in the continuous human and animal cell cultures. The model of technology for the rotavirus vaccine, based on the industrial strain of rotavirus, is developed for its cultivation in continuous cell culture Vero. Specifically, the method of pseudo-suspension cultivation on DEAE Sephadex A-50 microcarriers is used to increase the surface area for cultivation of Vero cells.

Shchurska K.O., Kuzminskiy Ye.V.

Biohydrogen Production Ways

P. 105–114.

Fig. 1. Tabl. 3. Refs.: 38 titles.

The present paper describes biohydrogen production methods according to the classification of energy sources used by microorganisms. We also conduct the comparative analysis of these processes. We describe the microorganism-producers, hydrogen formation reaction and optimal environmental parameters for every method of biohydrogen production. All these methods have their advantages and disadvantages. Crucially, we define the most optimal biohydrogen production way. Photobiological hydrogen production and biomass production should take place on the first stage. The formed biomass should be fermented on the second dark stage and its products will be used to produce biohydrogen by the bioelectrochemical method.

Arhipova V.V., Smotrayev R.V.

The Investigation of Calcium Carbonate Precipitation with Simultaneous Hydrolysis Process of Carbamide in Aqueous Solutions of Calcium Chloryede

P. 115–118.

Fig. 4. Tabl. 2. Refs.: 9 titles.

We reveal that the tiff with hexagonal particles is formed at the controllable slow precipitation from calcium chloride solution with carbamide. We also figure out the kinetic characteristics of the reaction: the velocity constants at various temperatures, the reaction order and the activation energy.

Izotov V.Yu.

The Dependence of Integral Capacity of Double-Layer Electric Capacitor on Potential

P. 119–122.

Fig. 1. Tabl. 1. Refs.: 6 titles.

The paper studies the dependence of supercapacitor’s integral capacity on potential. We prove that the capacity as a linear function depends on the potential. We also show that the neglect of the potential’s influence on supercapacitor capacity can result in significant failures at calculation of energy intensity.

Kovalenko I.V., Lisin V.I., Andriiko A.A.

The Physical and chemical properties of composite systems with nonconductive oxide fillers

P. 123–128.

Fig. 2. Tabl. 1. Refs.: 24 titles.

In this paper, we analyze the body of scientific data on the effect of disperse filling of salt systems by oxide materials. Emphasized here is the nano-sized TiO2 as a disperse filler. The experimental data shows that the 5,9 V electrochemical window of pure and filled ionic liquid PYR14TFSI with the increased (to 30 %) electrical conductivity is stable. Such windows allow PYR14TFSI filled by nano-sized TiO2 to be used with very strong reducing and oxidizing electrode agents, yielding high cell voltage. Additionally, we consider various models for the disperse filling effect and discuss this effect on supercooling and glass transition.

Kosogin O.V., Linyucheva O.V.

The Electrochemical Sensor of Carbon Dioxide with Manganese Dioxide Working Electrode

P. 129–134.

Fig. 6. Tabl. 1. Refs.: 13 titles.

Relying on the electrochemical cell with the manganese dioxide gas-diffusion electrode, we develop the titanium-based galvanic-type sensor of carbon dioxide. Furthermore, we define the effect of such factors as the amount of manganese dioxide on the working electrode, the pH of solution in the sensor, the magnitude of diffusion resistance (the size of gas diffusion window), the internal resistance of electrochemical cell and the resistance of load resistor on the metrological characteristics of the sensor (the normalization of the current signal of sensor and the transient time t0,9).

Fialkov Yu.Ya., Kirsenko T.V., Kamens’ka T.A.

The Effect of Temperature Change of the Solvent Properties on the Termodynamic Characteristics of Chemical Equilibrium in Solutions

P. 135–140.

Tabl. 6. Refs.: 12 titles.

This paper proposes a novel approach to dividing thermodynamic characteristics of the chemical equilibrium in solutions, calculated from the polytherm of equilibrium constants. The first component of the equilibrium constant characterizes the process; the second one is caused by the temperature change of the solvent properties. This approach suggests that the extreme on polytherm equilibrium constants appear not because of the change in the nature of the process in the solution, but because of the temperature variation of the solvent permittivity.

Miroshnichenko J.A., Beznosyk Yu.O., Smirnova O.V., Zub Y.L., Leszczynski J.

Quantum-Chemical Modelling of Functionalized Silica Surface

P. 141–145.

Fig. 4. Tabl. 1. Refs.: 13 titles.

Based on the quantum chemical modeling of silica surface (DFT (B3LYP/6-31G (d, p))) functionalized with phosphorus- and thiourea-containing groups [≡Si(CH2)2P(O)(OC2H5)2, ≡Si(CH2)2P(O)OH2 and ≡Si(CH2)3NHC(S) NHC2H5], we show that hydrogen bonds can be formed between donor and silanol groups in the T2 structural unit both in the presence and absence of water molecule. The functionalized xerogel surface was modeled by (HO)2Si(OH)R′ fragments, where R′ = –(CH2)2P(O)(OC2H5)2, –(CH2)2P(O)(OH)2 or –(CH2)3NHC(S)NHC2H5. Specifically, geometry is optimized and spectra frequencies are calculated for these fragments. We also show that the absorption band of stretching vibrations ν (PO) in the calculated IR spectra for fragments with an intermolecular hydrogen bond is slightly shifted to lower frequencies, but it significantly increases with the appearance of a water molecule.

Prokofyeva G.M., Sudarushkina T.V., Savicheva K.Yu., Olifer O.M.

Development of Environmentally Safe Technical Detergent

P. 146–149.

Fig. 6. Refs.: 6 titles.

This study demonstrates the possibility of introducing BG-10 surfactant into the technical detergent. We do the research into BG-10–Ferrum (III), BG-10–МЕА–Ferrum (III), BG-10–МЕА–Leik–Ferrum (III) as a pollution ingredient interaction that can cause redeposition on the surface. Finally, we determine the corrosion activity and foaming ability of BG-10.